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ERO1L induces macrophage infiltration. A IHC staining showing F4/80 + , CD86 + , and CD206 + macrophage infiltration in the KC and KEC pancreas tissues harvested from the recovery phase. Scale bar: 50 μm. B In the recovery phase, F4/80 + , CD86 + , and CD206 + macrophage infiltration in the DMSO-treated or EN460-treated KC pancreas tissues were analyzed by IHC method. Scale bar: 50 μm. C Analyzing the correlation between ERO1L expression and various immune cells using the TCGA database. D Representative IHC staining images of ERO1L and CD68 in the pancreatic cancer specimens from Ren Ji cohort, scale bar: 100 μm. The correlation curve shows the correlation between ERO1L H-score and macrophage marker (CD68) in pancreatic cancer tissues. E Chemotaxis assay and statistical results of human macrophages (THP-1) treated with conditioned medium from sh-ERO1L and shCtrl pancreatic cancer cells (Capan-2 and MiaPaCa-2). **** P < 0.0001

Journal: Cell & Bioscience

Article Title: ERO1L induces macrophage infiltration to potentiate Kras G12D and inflammation-induced pancreatic tumorigenesis via oxidative protein folding of CCL2

doi: 10.1186/s13578-026-01601-3

Figure Lengend Snippet: ERO1L induces macrophage infiltration. A IHC staining showing F4/80 + , CD86 + , and CD206 + macrophage infiltration in the KC and KEC pancreas tissues harvested from the recovery phase. Scale bar: 50 μm. B In the recovery phase, F4/80 + , CD86 + , and CD206 + macrophage infiltration in the DMSO-treated or EN460-treated KC pancreas tissues were analyzed by IHC method. Scale bar: 50 μm. C Analyzing the correlation between ERO1L expression and various immune cells using the TCGA database. D Representative IHC staining images of ERO1L and CD68 in the pancreatic cancer specimens from Ren Ji cohort, scale bar: 100 μm. The correlation curve shows the correlation between ERO1L H-score and macrophage marker (CD68) in pancreatic cancer tissues. E Chemotaxis assay and statistical results of human macrophages (THP-1) treated with conditioned medium from sh-ERO1L and shCtrl pancreatic cancer cells (Capan-2 and MiaPaCa-2). **** P < 0.0001

Article Snippet: Following a 12-h incubation of the THP-1 cells, the migrated cells were treated with 2 μM Calcein-AM (MCE, HY-D0041) for 15 min and subsequently observed under a fluorescence microscope.

Techniques: Immunohistochemistry, Expressing, Marker, Chemotaxis Assay